Q1 Research Application · Frontier Focus

Microwave-assisted aqueous two-phase extraction of phenolic compounds from Ribes nigrum L. and its antibacterial effect on foodborne pathogens

This Food Control paper (2021) is indexed as a Xianghu equipment application case for XH-800S; key results include Sample purity 80.50 ± 0.26%.

View DOI Back to Library Chinese Solution
Paper ID 358
Application Focus Microwave-assisted extraction
Key Result Sample purity 80.50 ± 0.26%
Core Condition Microwave power 600 W
Paper ID
358
Journal
Food Control
Impact Factor
4.258
CAS Zone
Zone 1
Year
2021
DOI
10.1016/j.foodcont.2020.107449
Equipment Model
XH-800S
Affiliations
Northeast Agricultural University
Research Directions
Microwave-assisted extraction

Fact Snapshot

  • Paper: Microwave-assisted aqueous two-phase extraction of phenolic compounds from Ribes nigrum L. and its antibacterial effect on foodborne pathogens
  • Equipment: XH-800S
  • Source: Food Control, 2021
  • Research direction: Microwave-assisted extraction
  • Core conditions: Microwave power 600 W and Time 10 min / 24 h / 8 h
  • Key results: Sample purity 80.50 ± 0.26%

Research Abstract

Microwave-assisted aqueous two-phase extraction of phenolic compounds from Ribes nigrum L. and its antibacterial effect on foodborne pathogens was published in Food Control (2021) and is indexed as a Xianghu Q1 application case for XH-800S. The source record connects it with Microwave-assisted extraction. Core operating conditions include Microwave power 600 W and Time 10 min / 24 h / 8 h. Key reported results include Sample purity 80.50 ± 0.26%.

Research Background and Problem

The application context is Microwave-assisted extraction. The equipment metadata identifies XH-800S, while the publication metadata records Food Control (2021). The affiliation record includes Northeast Agricultural University. The recorded DOI is 10.1016/j.foodcont.2020.107449.

Equipment Use and Experimental Conditions

ItemParameter
Microwave power600 W
Time10 min / 24 h / 8 h

Key Result

Sample purity 80.50 ± 0.26%
MetricResult
Sample purity80.50 ± 0.26%

Evidence Details

Yield evidence

Yield evidence: source values include 31.40%, 13.72%, 58.60 ± 1.97 mg, 80.50 ± 0.26%. Entities: XH-800C, AB-8.

Source evidence

Based on the pre-experiment data of MAE, the optimal conditions were determined as liquid to material ratio of 70:1 mL/g (v/w), extraction temperature of 65 °C, extraction time of 10 min and microwave power of 600 W.

Source evidence

When incubated with C3G (2 MIC) for 8 h, LDH activities increased from 0.32 ± 0.03 (control) to 2.21 ± 0.05 U/mL, protein contents increased from 6.28 ± 0.44 (control) to 46.67 ± 0.83 μg/mL, and OD260nm values increased from 0.077 (control) to 0.29, respectively.

Source evidence

The yields of phenolic compounds extracted with SE, UAE and MAE were 26.32 ± 0.64, 34.98 ± 0.83 and 40.19 ± 1.37 mg/g, respectively, which were lower than that of MA-ATPE (58.60 ± 1.97 mg/g).

Source evidence

MIC values obtained revealed that C3G presented the highest antibacterial activity against S.aureus with the MIC of 2.0 mg/mL, while the MICs of K3R, rutin and hyperoside were all 4.0 mg/mL.

Condition evidence

Time: 10 min / 24 h / 8 h

Source evidence

At the concentration of 4 MIC, the inhibition rate on biofilm formation was 71.32 ± 1.05%, and the IC50 value was 1.71.

Additional source evidence

Additional source evidence: source values include 2.5 mg/mL, 5 mg/mL.

Mechanism / Method Highlights

  • Method context: Microwave power 600 W and Time 10 min / 24 h / 8 h.
  • Yield evidence: source values include 31.40%, 13.72%, 58.60 ± 1.97 mg, 80.50 ± 0.26%. Entities: XH-800C, AB-8
  • Source evidence: Based on the pre-experiment data of MAE, the optimal conditions were determined as liquid to material ratio of 70:1 mL/g (v/w), extraction temperature of 65 °C, extraction time of 10 min and microwave power of 600 W
  • Source evidence: When incubated with C3G (2 MIC) for 8 h, LDH activities increased from 0.32 ± 0.03 (control) to 2.21 ± 0.05 U/mL, protein contents increased from 6.28 ± 0.44 (control) to 46.67 ± 0.83 μg/mL, and OD260nm values increased from 0.077 (control) to 0.29, respectively
  • Reported outcome: Sample purity 80.50 ± 0.26%.

Application Value

  • Provides a peer-reviewed SoarNova / Xianghu Q1 application case for XH-800S.
  • Supports English discovery around Microwave-assisted extraction.
  • Preserves quantitative result evidence: Sample purity 80.50 ± 0.26%.
  • Maintains source-level evidence details: Yield evidence: source values include 31.40%, 13.72%, 58.60 ± 1.97 mg, 80.50 ± 0.26%. Entities: XH-800C, AB-8, Source evidence: Based on the pre-experiment data of MAE, the optimal conditions were determined as liquid to material ratio of 70:1 mL/g (v/w), extraction temperature of 65 °C, extraction time of 10 min and microwave power of 600 W, Source evidence: When incubated with C3G (2 MIC) for 8 h, LDH activities increased from 0.32 ± 0.03 (control) to 2.21 ± 0.05 U/mL, protein contents increased from 6.28 ± 0.44 (control) to 46.67 ± 0.83 μg/mL, and OD260nm values increased from 0.077 (control) to 0.29, respectively, and Source evidence: The yields of phenolic compounds extracted with SE, UAE and MAE were 26.32 ± 0.64, 34.98 ± 0.83 and 40.19 ± 1.37 mg/g, respectively, which were lower than that of MA-ATPE (58.60 ± 1.97 mg/g).

Related Equipment

XH-800SE Microwave Hydrothermal Synthesizer
XH-800SE Microwave Hydrothermal Synthesizer

FAQ

Which Xianghu instrument is covered by this page?
The structured source records XH-800S for this paper.
What research direction does this page support?
The source tags this paper under Microwave-assisted extraction.
Which publication does this case come from?
It comes from Food Control (2021), DOI 10.1016/j.foodcont.2020.107449.
Citation
Microwave-assisted aqueous two-phase extraction of phenolic compounds from Ribes nigrum L. and its antibacterial effect on foodborne pathogens
Food Control, 2021
DOI: 10.1016/j.foodcont.2020.107449